微乳液
超顺磁性
纳米颗粒
氧化铁纳米粒子
磁性纳米粒子
氧化铁
化学
吸附
材料科学
化学工程
表面改性
核化学
磁化
分析化学(期刊)
纳米技术
色谱法
物理化学
有机化学
肺表面活性物质
工程类
物理
磁场
量子力学
生物化学
作者
Chuka Okoli,Margarita Sánchez-Domínguez,Magali Boutonnet,Sven Järås,Concepción Civera,Conxita Solans,Gunaratna Rajarao Kuttuva
出处
期刊:Langmuir
[American Chemical Society]
日期:2012-05-24
卷期号:28 (22): 8479-8485
被引量:139
摘要
Magnetic iron oxide nanoparticles (MION) for protein binding and separation were obtained from water-in-oil (w/o) and oil-in-water (o/w) microemulsions. Characterization of the prepared nanoparticles have been performed by TEM, XRD, SQUID magnetometry, and BET. Microemulsion-prepared magnetic iron oxide nanoparticles (ME-MION) with sizes ranging from 2 to 10 nm were obtained. Study on the magnetic properties at 300 K shows a large increase of the magnetization ∼35 emu/g for w/o-ME-MION with superparamagnetic behavior and nanoscale dimensions in comparison with o/w-ME-MION (10 emu/g) due to larger particle size and anisotropic property. Moringa oleifera coagulation protein (MOCP) bound w/o- and o/w-ME-MION showed an enhanced performance in terms of coagulation activity. A significant interaction between the magnetic nanoparticles and the protein can be described by changes in fluorescence emission spectra. Adsorbed protein from MOCP is still retaining its functionality even after binding to the nanoparticles, thus implying the extension of this technique for various applications.
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