拟南芥
WRKY蛋白质结构域
生物化学
他马汀
蔗糖
突变体
NPR1
拟南芥
生物
水杨酸
基因表达
基因
医学
内科学
利钠肽
心力衰竭
作者
Marie-Christine Thibaud,Sandrine Gineste,Laurent Nussaume,Christophe Robaglia
标识
DOI:10.1016/j.plaphy.2003.10.012
摘要
Pathogenesis-related (PR) protein-coding gene expression was studied in Arabidopsis thaliana grown in liquid medium in the presence of sugars (sucrose or glucose). PR protein transcripts accumulated in the presence of sugar in the medium. A potential effect linked to osmolarity changes induced by sugar addition in the medium was ruled out using osmotica (NaCl or polyethylene glycol). Two major proteins were purified from the culture medium and found to be homologous to A. thaliana PR-2 (acidic form of beta-1, 3-glucanase) and PR-5 (thaumatin-like PR-protein). The expression of the corresponding genes was increased in the presence of sucrose and was detected exclusively in the green parts of the plant. The use of mutants and transgenic plants of A. thaliana indicated that salicylic acid (SA) was involved in the sugar-dependent activation of these PR protein-coding genes. Activation of the PR-2-coding gene was demonstrated not to be hexokinase-dependent and to be linked to a sugar metabolite acting as an internal signal as shown with non-metabolizable sugars, which were inefficient for the induction of the PR-2-coding gene. Moreover, the activation of this gene occurred in the npr1 mutant suggesting that the sugar signal acts either downstream or independently of NPR1.
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