复印机
细胞生物学
囊泡转运蛋白
高尔基体
膜泡运输蛋白质类
复印件
化学
生物
小泡
内质网
分泌途径
生物化学
膜
细胞质
液泡
液泡蛋白分选
作者
Jane Yang,Carmen Valente,Roman Polishchuk,Gabriele Turacchio,Emilie Layre,D. Branch Moody,Christina C. Leslie,Michael H. Gelb,William J. Brown,Daniela Corda,Alberto Luini,Victor Hsu
摘要
Intracellular transport occurs through two general types of carrier, either vesicles or tubules. Coat proteins act as the core machinery that initiates vesicle formation, but the counterpart that initiates tubule formation has been unclear. Here, we find that the coat protein I (COPI) complex initially drives the formation of Golgi buds. Subsequently, a set of opposing lipid enzymatic activities determines whether these buds become vesicles or tubules. Lysophosphatidic acid acyltransferase-γ (LPAATγ) promotes COPI vesicle fission for retrograde vesicular transport. In contrast, cytosolic phospholipase A2-α (cPLA2α) inhibits this fission event to induce COPI tubules, which act in anterograde intra-Golgi transport and Golgi ribbon formation. These findings not only advance a molecular understanding of how COPI vesicle fission is achieved, but also provide insight into how COPI acts in intra-Golgi transport and reveal an unexpected mechanistic relationship between vesicular and tubular transport.
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