视网膜色素上皮
视网膜
视网膜
颜料
壳体(结构)
芯(光纤)
生物医学工程
上皮
CD44细胞
材料科学
生物物理学
细胞生物学
眼科
医学
生物
病理
细胞
化学
复合材料
生物化学
神经科学
有机化学
作者
Li Gan,J. Wang,Yanan Zhao,Dan Chen,Chunliu Zhu,Jianping Liu,Yong Gan
出处
期刊:Biomaterials
[Elsevier]
日期:2013-08-01
卷期号:34 (24): 5978-5987
被引量:75
标识
DOI:10.1016/j.biomaterials.2013.04.035
摘要
Retinal inflammation, a common process of posterior ocular diseases, may lead to severe vision loss or even blindness. Retinal pigment epithelium (RPE) cells are generally considered as the therapeutic target of inflammation pathogenesis. However, the lack of retina-specific distribution for general intravitreous drug delivery systems makes the anti-inflammation treatment inefficient. In the present study, a hyaluronan (HA)-modified core–shell liponanoparticles (HA-LCS-NPs) was designed to improve the treatment efficiency by increasing RPE-targeted distribution. Our in vitro RPE cell uptake study showed that a higher HA grafting density (5.8%) and a higher molecular weight (200–400 kDa) modification of HA improved the intracellular uptake of HA-LCS-NPs. In addition, in vivo distribution evaluation in experimental autoimmune uveitis (EAU) rats revealed that HA-LCS-NPs could specifically target RPE cells through the interaction between the CD44 receptor and the HA ligand, while chitosan nanoparticles (CS-NPs) were limited to the vitreous cavity and the core–shell liponanoparticles (LCS-NPs) only reached the inner layers of the retina. At 7 d post-injection, approximately 75% of the fluorescence of HA-LCS-NPs still remained in the RPE/choroid. In conclusion, HA-LCS-NPs might present a promising intraocular drug delivery system to achieve RPE-targeted distribution and prolonged intraocular residence.
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