磷脂酶
交易激励
心理压抑
生物
抄写(语言学)
长终端重复
分子生物学
染色体易位
体外
病毒学
转录因子
细胞生物学
基因
基因表达
磷脂酰丝氨酸
遗传学
磷脂
哲学
语言学
膜
作者
Shuichi Kusano,Yoshito Eizuru
标识
DOI:10.1016/j.bbrc.2013.02.098
摘要
Human phospholipid scramblase 1 (PLSCR1) is an interferon (IFN)-stimulated gene and possesses an IFN-mediated antiviral function. We show here that PLSCR1 directly interacts with human immunodeficiency virus type-1 (HIV-1) Tat. This interaction occurs both in vitro and in vivo through amino acids 160-250 of PLSCR1. Overexpression of PLSCR1 efficiently represses the Tat-dependent transactivation of the HIV-1 long terminal repeat (LTR) and reduces the nuclear translocation of Tat. In addition, shRNA-mediated suppression of endogenous PLSCR1 expression enhances the levels of gag mRNA in an HIV-1-infected T-cell line. These findings indicate that PLSCR1 negatively regulates the Tat-dependent transactivation of the HIV-1 LTR during HIV-1 infection.
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