粘附
一氧化氮
化学
A549电池
细胞粘附分子
细胞粘附
细胞生物学
细胞内
生物物理学
细胞
生物化学
生物
有机化学
作者
Madeeha Aqil,Kim M. Elseth,Ashok Arjunakani,Philip Nebres,Courtney Amegashie,Devang H. Thanki,Premal B. Desai,James A. Radosevich
出处
期刊:Tumor Biology
[SAGE]
日期:2014-12-11
卷期号:36 (3): 1871-1879
被引量:3
标识
DOI:10.1007/s13277-014-2789-9
摘要
The migration and adhesion properties of tumors affect their metastatic rate. In the present study, we investigated carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 1, 5, and 6 expression in high nitric oxide (HNO)-adapted lung cancer cells compared to parent cells. We observed high transcript levels of CEACAM 1 (4S, 4L), CEACAM 5, and CEACAM 6 in HNO cells compared to parent cells. However, the surface expression was low in HNO cells. Interestingly, the intracellular protein levels were high for these three CEACAMs. We confirmed these results with immunohistochemical experiments. Further, the adhesion and migration assays showed reduced clumping in HNO-adapted A549 (A549-HNO) cells and faster migration rates, respectively. These results document the altered adhesion and migration properties of cells adapted to HNO. Further, our studies also indicate a dynamic regulation of CEACAM protein expression and surface transport in HNO cells.
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