Physical Properties of Beeswax-based Oleogel-emulsion as a Delivery System of Probiotics

This research aims to develop an emulsion-oleogel delivery system for probiotics. Different combinations of oleogelators (beeswax: ethylcellulose, monopalmitin, or glycerol monostearate) were prepared in different rations (10:0, 7:3, 5:5, 3:7, 0:10). The oleogels with 10% oleogelators combined to structure soybean oil. The oleogels were analyzed by oil binding capacity, texture, rheology, PLM (polarized light microscopy), and DSC (Differential Scanning Calorimetry). The oleogel with (7:3) beeswax: glycerol monostearate as oleogelators shows the desired stability range between 45-50℃ and is suitable for probiotics delivery system based on the physical properties. The texture showed a firmness of 204.3±13.7g and a toughness of 502.4±50.0 g. The oleogel retained 100% of the oil after 60 days. The melting curves presented Ton (Onset Temperature) of 37.5±0.4℃, with a Tp (peak temperature) at 51.6±0.3℃, and an DH of 8.4±0.1J/g). The emulsion-oleogels were prepared using the 7:3 ratio (beeswax and glyceryl monostearate). The emulsion experiments tested different ratios (0.5, 1, 1.5, 2%) of emulsifiers (Tween 80, Phospholipon, and Lecithin) in three ratios of ionized water (5, 10, 15%). The visual analysis indicates that the emulsions using Phospholipon had minimum phase separation and better stability than the other emulsifiers. The results show that oleogel emulsions prepared with 1.5% Phospholipon in a 10 % water phase are a stable probiotic delivery system.