Bioinformatics analysis and consistency verification of a novel tuberculosis vaccine candidate HP13138PB

表位 免疫原性 埃利斯波特 免疫系统 肽疫苗 抗原性 免疫学 抗原 病毒学 医学 肺结核 接种疫苗 结核病疫苗 生物 T细胞 结核分枝杆菌 病理
作者
Peng Cheng,Fan Jiang,Guiyuan Wang,Jie Wang,Yong Xue,Liang Wang,Wenping Gong
出处
期刊:Frontiers in Immunology [Frontiers Media SA]
卷期号:14
标识
DOI:10.3389/fimmu.2023.1102578
摘要

With the increasing incidence of tuberculosis (TB) and the shortcomings of existing TB vaccines to prevent TB in adults, new TB vaccines need to be developed to address the complex TB epidemic.The dominant epitopes were screened from antigens to construct a novel epitope vaccine termed HP13138PB. The immune properties, structure, and function of HP13138PB were predicted and analyzed with bioinformatics and immunoinformatics. Then, the immune responses induced by the HP13138PB were confirmed by enzyme-linked immunospot assay (ELISPOT) and Th1/Th2/Th17 multi-cytokine detection kit.The HP13138PB vaccine consisted of 13 helper T lymphocytes (HTL) epitopes, 13 cytotoxic T lymphocytes (CTL) epitopes, and 8 B-cell epitopes. It was found that the antigenicity, immunogenicity, and solubility index of the HP13138PB vaccine were 0.87, 2.79, and 0.55, respectively. The secondary structure prediction indicated that the HP13138PB vaccine had 31% of α-helix, 11% of β-strand, and 56% of coil. The tertiary structure analysis suggested that the Z-score and the Favored region of the HP13138PB vaccine were -4.47 88.22%, respectively. Furthermore, the binding energies of the HP13138PB to toll-like receptor 2 (TLR2) was -1224.7 kcal/mol. The immunoinformatics and real-world experiments showed that the HP13138PB vaccine could induce an innate and adaptive immune response characterized by significantly higher levels of cytokines such as interferon-gamma (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and IL-10.The HP13138PB is a potential vaccine candidate to prevent TB, and this study preliminarily evaluated the ability of the HP13138PB to generate an immune response, providing a precursor target for developing TB vaccines.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
一路硕博完成签到,获得积分10
1秒前
细腻的怀蕊完成签到 ,获得积分10
1秒前
零零tube发布了新的文献求助20
1秒前
英俊的铭应助科研通管家采纳,获得10
2秒前
桐桐应助科研通管家采纳,获得10
2秒前
2秒前
FashionBoy应助科研通管家采纳,获得10
2秒前
2秒前
2秒前
完美世界应助科研通管家采纳,获得10
2秒前
orixero应助科研通管家采纳,获得10
2秒前
2秒前
2秒前
粗心的菀完成签到 ,获得积分10
2秒前
乐观期待完成签到,获得积分10
3秒前
cwy完成签到,获得积分20
4秒前
NexusExplorer应助宇子采纳,获得10
4秒前
5秒前
spring完成签到 ,获得积分10
6秒前
6秒前
DYQ完成签到,获得积分10
7秒前
8秒前
沉默寄风完成签到,获得积分10
8秒前
uiwh发布了新的文献求助10
9秒前
NicheFactor完成签到,获得积分10
9秒前
Wcy发布了新的文献求助10
10秒前
12秒前
大模型应助白菜也挺贵采纳,获得10
13秒前
NexusExplorer应助DYQ采纳,获得10
13秒前
爱洗澡的鱼完成签到 ,获得积分10
13秒前
论英雄完成签到 ,获得积分10
15秒前
Wcy完成签到,获得积分10
16秒前
思源应助栩栩采纳,获得10
16秒前
17秒前
17秒前
chemlixy完成签到,获得积分20
18秒前
xxy完成签到,获得积分10
19秒前
19秒前
希望天下0贩的0应助庾稀采纳,获得10
21秒前
22秒前
高分求助中
Teaching Social and Emotional Learning in Physical Education 900
Plesiosaur extinction cycles; events that mark the beginning, middle and end of the Cretaceous 800
Recherches Ethnographiques sue les Yao dans la Chine du Sud 500
Two-sample Mendelian randomization analysis reveals causal relationships between blood lipids and venous thromboembolism 500
Chinese-English Translation Lexicon Version 3.0 500
Wisdom, Gods and Literature Studies in Assyriology in Honour of W. G. Lambert 400
薩提亞模式團體方案對青年情侶輔導效果之研究 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 有机化学 工程类 生物化学 纳米技术 物理 内科学 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 电极 光电子学 量子力学
热门帖子
关注 科研通微信公众号,转发送积分 2392163
求助须知:如何正确求助?哪些是违规求助? 2096788
关于积分的说明 5282845
捐赠科研通 1824347
什么是DOI,文献DOI怎么找? 909852
版权声明 559895
科研通“疑难数据库(出版商)”最低求助积分说明 486223