生物膜
单线态氧
金属有机骨架
微生物学
化学
金黄色葡萄球菌
纳米颗粒
纳米技术
细菌
材料科学
生物
氧气
有机化学
吸附
遗传学
作者
Renfei Wu,Tao Yu,Dong Han,Rui Shi,Guimei Jiang,Yijin Ren,Henny C. van der Mei,Henk J. Busscher,Jian Liu
出处
期刊:ACS Nano
[American Chemical Society]
日期:2023-01-24
卷期号:17 (3): 2328-2340
被引量:5
标识
DOI:10.1021/acsnano.2c09008
摘要
Eradication of infectious biofilms is becoming increasingly difficult due to the growing number of antibiotic-resistant strains. This necessitates development of nonantibiotic-based, antimicrobial approaches. To this end, we designed a heterocatalytic metal-organic framework composed of zirconium 1,4-dicarboxybenzene (UiO-66) with immobilized Pt nanoparticles (Pt-NP/UiO-66). Pt-NP/UiO-66 enhanced singlet-oxygen generation compared with Pt nanoparticles or UiO-66, particularly in an acidic environment. Singlet-oxygen generation degraded phosphodiester bonds present in eDNA gluing biofilms together and therewith dispersed biofilms. Remaining biofilms possessed a more open structure. Concurrently, Pt-NP/UiO-66 stimulated macrophages to adapt a more M1-like, "fighting" phenotype, moving faster toward their target bacteria and showing increased bacterial killing. As a combined effect of biofilm dispersal and macrophage polarization, a subcutaneous Staphylococcus aureus biofilm in mice was more readily eradicated by Pt-NP/UiO-66 than by Pt nanoparticles or UiO-66. Therewith, heterocatalytic Pt-NP/UiO-66 metal-organic frameworks constitute a nonantibiotic-based strategy to weaken protective matrices and disperse infectious biofilms, while strengthening macrophages in bacterial killing.
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