Physicochemical, structural and in vitro gastrointestinal tract release properties of ι-carrageenan/sodium caseinate synbiotic microgels produced by double-crosslinking with calcium ions and transglutaminase

The aim of this study was to develop ι-carrageenan (ιC)/sodium caseinate (NaCas) synbiotic microgels loading Lacticasebacillus paracasei produced by double-crosslinking with calcium ions and different concentrations (0, 5, 10, and 15 U/g protein) of transglutaminase (TGase). The synbiotic microgels were coated/filled with pectic oligosaccharide (POS). Field emission scanning electron microscope (FE-SEM) and X-ray diffraction (XRD) analyses indicated that L. paracasei was successfully microencapsulated in synbiotic microgels. In Fourier transform infrared (FT-IR) analysis, the new formation of covalent and ionic crosslinking was observed in double-crosslinked synbiotic microgels. The encapsulation efficiency of L. paracasei was significantly increased from 87.82 to 97.68 % by increasing the concentration of TGase from 0 to 15 U/g protein, respectively. After exposure to simulated gastric fluid for 2 h and simulated intestinal fluid for 4 h, the survival rate of L. paracasei was significantly increased as the concentration of TGase increased.