Lamp Primer Designing Software: The Overview

The loop-mediated isothermal amplification (LAMP) of DNA is considered as the most promising method for DNA diagnostics of infectious diseases in a “point-of-care testing” format. Currently, the online services and downloadable programs have been developed for design of LAMP primers, some of them (freely available) are overviewed here. Among them, the PrimerExplorer software appears to be mostly used, followed by the NEB LAMP Primer Design Tool that is steadily gaining popularity. The other non-commercial programs such as GLAPD, MorphoCatcher, LAMPrimer iQ, and LAVA have been utilized for LAMP primer design much more rarely. The advantages and disadvantages of the currently existing free software for the construction of LAMP primers, alongside with directions for its further development, are discussed.