炎症
免疫印迹
化学
活性氧
脂多糖
氧化铈
纳米颗粒
纳米材料
分子生物学
免疫学
纳米技术
材料科学
生物化学
医学
生物
基因
催化作用
作者
Ying Lü,Xiaomin Xia,Zhipeng Niu,Ke Wang,Jie Liu,Xue Li
标识
DOI:10.3389/fimmu.2024.1344098
摘要
Inflammatory responses, especially chronic inflammation, are closely associated with many systemic diseases. There are many ways to treat and alleviate inflammation, but how to solve this problem at the molecular level has always been a hot topic in research. The use of nanoparticles (NPs) as anti-inflammatory agents is a potential treatment method. We synthesized new hollow cerium oxide nanomaterials ( h CeO 2 NPs) doped with different concentrations of Cu 5.4 O NPs [the molar ratio of Cu/(Ce + Cu) was 50%, 67%, and 83%, respectively], characterized their surface morphology and physicochemical properties, and screened the safe concentration of h CeO 2 @Cu 5.4 O using the CCK8 method. Macrophages were cultured, and P.g -lipopolysaccharide-stimulated was used as a model of inflammation and co-cultured with h CeO 2 @Cu 5.4 O NPs. We then observe the effect of the transcription levels of CTSB, NLRP3, caspase-1, ASC, IL-18, and IL-1β by PCR and detect its effect on the expression level of CTSB protein by Western blot. The levels of IL-18 and IL-1β in the cell supernatant were measured by enzyme-linked immunosorbent assay. Our results indicated that h CeO 2 @Cu 5.4 O NPs could reduce the production of reactive oxygen species and inhibit CTSB and NLRP3 to alleviate the damage caused by the inflammatory response to cells. More importantly, h CeO 2 @Cu 5.4 O NPs showed stronger anti-inflammatory effects as Cu 5.4 O NP doping increased. Therefore, the development of the novel nanomaterial h CeO 2 @Cu 5.4 O NPs provides a possible new approach for the treatment of inflammatory diseases.
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