适体
分析物
材料科学
分子
纳米技术
模块化设计
小分子
计算机科学
组合化学
光电子学
化学
有机化学
生物
色谱法
遗传学
操作系统
作者
Amani A. Hariri,Alyssa P. Cartwright,Constantin Dory,Yasser Gidi,Steven Yee,Ian A. P. Thompson,Kaiyu Fu,Kailu Yang,Diana Wu,Nicolò Maganzini,Trevor Feagin,Brian E. Young,Behrad Habib Afshar,Michael Eisenstein,Michel J. F. Digonnet,Jelena Vučković,H. Tom Soh
标识
DOI:10.1002/adma.202304410
摘要
Abstract Aptamers are a promising class of affinity reagents because signal transduction mechanisms can be built into the reagent, so that they can directly produce a physically measurable output signal upon target binding. However, endowing the signal transduction functionality into an aptamer remains a trial‐and‐error process that can compromise its affinity or specificity and typically requires knowledge of the ligand binding domain or its structure. In this work, a design architecture that can convert an existing aptamer into a “reversible aptamer switch” whose kinetic and thermodynamic properties can be tuned without a priori knowledge of the ligand binding domain or its structure is described. Finally, by combining these aptamer switches with evanescent‐field‐based optical detection hardware that minimizes sample autofluorescence, this study demonstrates the first optical biosensor system that can continuously measure multiple biomarkers (dopamine and cortisol) in complex samples (artificial cerebrospinal fluid and undiluted plasma) with second and subsecond‐scale time responses at physiologically relevant concentration ranges.
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