Shinella sedimenti sp. nov., isolated from sediment of Zhairuo Island located in the East China Sea

A Gram-negative, rod-shaped and aerobic bacterial strain B3.7^T, was isolated from the sediment of Zhairuo Island, Zhoushan city, Zhejiang Province, PR China. Maximum growth of strain B3.7^T was observed at 30 °C when cultured in a medium containing 0.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain B3.7^T belonged to the genus Shinella; it showed the highest sequence similarity of 98.47 % to Shinella kummerowiae CCBAU 25048^T. The average nucleotide identity and digital DNA-DNA hybridization values between strain B3.7^T and its reference strains were 82.9-84.2 % and 26.1-27.3 %, respectively. Chemotaxonomic analysis indicated that the sole respiratory quinone was Q-10 and the predominant cellular fatty acids were C_{19 : 0} cyclo ω8c, C_{16 : 0}, C_{18 : 1} ω7c 11-methyl and summed feature 8 (C_{18 : 1} ω7c and/or C_{18 : 1} ω6c). The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids and two unidentified aminolipids. Collectively, strain B3.7^T can be considered to represent a novel species, for which the name Shinella sedimenti sp. nov. is proposed. The type strain is B3.7^T (=MCCC 1K07163^T=LMG 32559^T).