Catalytic mechanisms of the 8–17 and 10–23 DNAzymes: shared mechanistic strategies

We discuss recent evidence from functional, crystallographic, NMR-based, and molecular dynamics studies that highlight how conserved guanine residues act as general bases, while hydrated metal ions contribute as general acids in both DNAzymes. By summarizing the latest advancements in the field, this review aims to provide a comprehensive perspective on the fundamental catalytic strategies employed by the 8-17 and the 10-23 DNAzymes and their relevance for future applications in nucleic acid-based catalysis.