Engineering strategies and applications of cyanobacterial exopolysaccharides: A review on past achievements and recent perspectives

Cyanobacteria are ideally suited for developing sustainable biological products but are underdeveloped due to a lack of genetic tools. Exopolysaccharide (EPS) is one of the essential bioproducts with widespread industrial applications. Despite their unique structural characteristics associated with distinct biological and physicochemical aspects, EPS from cyanobacteria has been underexplored. However, it is expected to accelerate in the near future due to the utilization of low-cost cyanobacterial platforms and readily available information on the structural data and specific features of these biopolymers. In recent years, cyanobacterial EPSs have attracted growing scientific attention due to their simple renewability, rheological characteristics, massive production, and potential uses in several biotechnology domains. This review focuses on the most recent research on potential new EPS producers and their distinct compositions responsible for novel biological activities. Additionally, nutritional and process parameters discovered recently for enhancing EPS production and engineering strategies applied currently to control the biosynthetic pathway for enhanced EPS production are critically highlighted. The process intensification of previously developed EPS extraction and purification processes from cyanobacterial biomass is also extensively explained. Furthermore, the newly reported biotechnological applications of cyanobacterial exopolysaccharides are also discussed.