重组DNA
蛋白质标签
亲和层析
蛋白质纯化
Myc标签
组氨酸
化学
标志标签
生物化学
靶蛋白
大肠杆菌
串联亲和纯化
色谱法
分子生物学
生物
氨基酸
融合蛋白
酶
基因
作者
Sinéad T. Loughran,Ronan T. Bree,Dermot Walls
出处
期刊:Methods in molecular biology
日期:2016-10-12
卷期号:: 275-303
被引量:20
标识
DOI:10.1007/978-1-4939-6412-3_14
摘要
His-tagging is the most widespread and versatile strategy used to purify recombinant proteins for biochemical and structural studies. Recombinant DNA methods are first used to engineer the addition of a short tract of poly-histidine tag (His-tag) to the N-terminus or C-terminus of a target protein. The His-tag is then exploited to enable purification of the "tagged" protein by Immobilized Metal Affinity Chromatography (IMAC). Here, we describe efficient procedures for the isolation of highly purified His-tagged target proteins from an E. coli host using IMAC.
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