Expressions of NF-κB and TLR4 in patients with diarrhea-predominant irritable bowel syndrome complicating with food intolerance and the influences of invigorating spleen decoctions on them

Objective It is to observe the expressions of NF-κB and TLR4 in patients with diarrhea-predominant irritable bowel syndrome(D-IBS) complicating with food intolerance and the influences of invigorating spleen decoctions on them.Methods 60 food intolerance patients with D-IBS were assigned randomly to treatment group and control group,each group had 30 cases.The treatment group was treated with invigorating spleen decoctions once a day,and the control group was treated with pinaverium bromide tablet(PVB) 50 mg three times a day.The curative effects were evaluated after 4 weeks.The expressions of NF-κB and TLR4 in duodenal mucosa were detected with immunohistochemistry method before and after treatment.Results The total effective rate of treatment group(93%) was significantly higher than that of control group(70%)(P0.01).The scores of main symptoms after treatment were significantly lower than that before treatment in both groups(P0.01).There was no significant difference between two groups in the expression of NF-κB and TLR4 pre-treatment.The expression of NF-κB and TLR4 in treatment group after therapy was significantly lower than that of control group(P0.05).Conclusion NF-κB and TLR4 play a role in the pathogenesis of D-IBS with food intolerance patients.The method of invigorating spleen is effective for treating D-IBS with food intolerance,which maybe due to regulate the expressions of NF-κB and TLR4 in duodenal mucosa.