Determination of lobetyolin in 7 species plants of Codonopsis by HPLC

OBJECTIVE To establish a method to determine the content of lobetyolin in 7 species plants of Codonopsis.METHODS RP-HPLC method was adopted.Hypersil C18(250 mm×4.6 mm,5 μm) with acetonitrile-water(20:80)as the mobile phase was used and DAD detection was set at 270 nm.RESULTS The standard curve was linear in the range of 0.025-6.180 μg·ml-1(r=0.9999)with the detection limit of 0.506 μg·ml-1.The average recovery was 98.74% with the RSD of 0.53%(n=6).26 specimens of 7 species plants of Codonopsis were analyzed.CONCLUSION The method is simple,rapid and reproducible and could be used for quality control of medicinal materials of Codonopsis.