Study on the antioxidation of safflower yellow

To explore the antioxidant activity of Safflower yellow(SY)and its main active ingredient,the inhibition of 2-deoxyribose degradation induced by hydroxyl radical and the ability of scavenging DPPH·was examined.Hydroxyl radical was produced through the Fenton reaction.The protection ability of SY and its main chemical components on 2-deoxyribose degradation induced by hydroxyl radical,and the ability of scavenging DPPH·were examined in vitro by UV spectrophotometer.At the same time,the mechanism of Safflower yellow B(SYB)inhibiting the degradation of 2-deoxyribose had been studied.Results showed that SY could inhibit the degradation of 2-deoxyribose induced by hydroxyl radical.SY could also eliminate DPPH·effectively.The IC50 was 256.79 μg/mL and 27.15 μg/mL respectively,and both of them showed a dose-effect relationship.Hydroxy safflower yellow A(HSYA)and SYB were two main active compounds in SY.The IC50 of inhibition 2-deoxyribose degradation was 220.68 μg/mL and 207.01 μg/mL,and the IC50 of scavenging DPPH·was 55.81 μg/mL and 41.25 μg/mL respectively.The results of mechanism test showed that,apart from direct scavenging the hydroxyl radicals produced by the Fenton reaction,SYB could also chelated with iron ions and block Fenton reaction.So SY had a significant antioxidation activity,while SYB and HSYA were main ingredients in SY.