This study determined Se content,total protein content and total Gypenosides content of gynostemma pentaphyllum which came from five different provinces and explored their correlation.Se content was determined by the hydride generation atomic fluorescence spectrometry(HG-AFS),kieldahl method was used to analyse the content of total protein,vanillin-perchloride acid colorimetry was used to determine gypenosides content.The highest Se content of gynostemma pentaphyllum in five provinces was in Xianfeng of Hubei,the highest protein content and total gypenosides content were gynostemma pentaphyllum of Qingcheng in Sichuan.The least contents of Se,total protein and total gypenosides of gynostemma pentaphyllum were in same place,Jinping in Guizhou.The contents of Se as well as total protein and total gypenosides and total protein of gynostemma pentaphyllum had significant correlation,correlation coefficient 0.910 and 0.919,respectively.In general,the total gypenosides content were also on the rise with the increase in Se content,but no significant correlation between them.The contents of Se,total protein and total gypenosides of gynostemma pentaphyllum were closely related and they were worth a further research.