Seed germination is a complicated process regulated by both internal and external factors. Light and temperature are two of the most critical environmental factors regulating seed germination. Recently extensive studies have been made to elucidate the molecular mechanism underling seed germination in response to light and temperature. Some key genes involved in light and temperature signal transduction have been identified and suggested that there is a complex crosstalk among the light,temperature and hormone signaling pathway. This paper will briefly summarize the progress on the molecular mechanism underling the regulation of seed germination by light and temperature signaling.