Determination of Chlorogenic Acid in Eucommia Ulmoides of Different Genotypes

[Objective]Chlorogenic acid content in eucommia ulmoides of different genotypes were detected.[Method]Ultrasonic was used to extract chorogenic acid.The content of Chlorogenic acid in Eucommia ulmoides at different genotraction was determined by HPLC method.The chromatographic column was xdb-c18(4.6 mm × 150.0 mm,5 μm) and the mobile phase consisted of acetonit rile-0.4% H3PO4 solution(10∶ 90) with a detection of 327 nm wavelength.[Result]The results showed that average content of chlorogenic in tetraploid eucommia ulmoides was 4.005 mg/g,and average of chlogenic in diploid eucommia ulmoides was 0.850 mg/g.[Conclusion]Multiple comparison found that the tetraploid eucommia ulmoides of chlorogenic acid were significantly higher than the diploid content of chlorogenic acid in leaves.