Preparation and characterization of monoclonal antibodies specific to the non-structure 1 protein of dengue virus serotype 2

To prepare and characterize monoclonal antibodies specific to the non-structure 1 protein of dengue virus serotype 2,Balb/c mice were immunized by recombinant dengue virus non-structure 1 protein with high immunogenicity and inactive dengue virus serotype 2.Splenocytes of immunized mice were fused with myeloma cells NS1 to produce hybridoma cell lines Secreting anti-non-structure 1 protein antibodies of dengue virus serotype 2.Enzyme-linked immunosorbent assay(ELISA)、immnofluorescence assay(IFA) and Western blot analysis were applied to identify specificity of antibodies.Fourteen strains of hybridoma cell lines steadily secreting antibodies against non-structure 1 protein of dengue virus serotype 2 were obtained.Two of those antibodies were IgG2b in subtype,the others were IgG1.These antibodies had characteristics of specific binding to dengue virus serotype 2 and recombinant non-structure 1 protein of dengue virus serotype 2.All of those strains were specific to non-structure 1 protein of dengue virus serotype 2 without detectable cross-reactivity with other three serotypes of dengue virus with IFA.It is evident that monoclonal antibodies specific to non-structure 1 protein of dengue virus serotype 2 with high activity and specificity had been successfully established.These results established a foundation for early diagnosis of dengue virus infection.