化学
荧光
检出限
过氧化氢
葡萄糖氧化酶
氧化物
吸光度
催化作用
核化学
生物传感器
分析化学(期刊)
光化学
无机化学
比色法
色谱法
有机化学
生物化学
物理
量子力学
作者
Yen-Linh Thi Ngo,Phi Luan Nguyen,Jayasmita Jana,Won Mook Choi,Jin Suk Chung,Seung Hyun Hur
标识
DOI:10.1016/j.aca.2020.11.023
摘要
A new strategy for the fluorescent and colorimetric sensing of hydrogen peroxide (H2O2) and glucose based on the metal oxide – carbon-dot hybrid structure was investigated. The sensing system is related to the catalytic oxidation reaction of glucose-by-glucose oxidase (GOx) to H2O2. In this study, a metal oxide hybrid with nitrogen-doped carbon dots (MFNCDs) that showed intrinsic peroxidase-like activity was synthesized and used as a catalyst instead of GOx to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to blue-emitting oxidized TMB (oxTMB) in the presence of hydrogen peroxide (H2O2). The fluorescence of MFNCDs/TMB at 405 nm was quenched in the presence of H2O2 through the inner filter effect (IFE) and electron transfer within MFNCDs, oxTMB, and glucose system. Therefore, the fluorescence and absorbance intensity can be applied to the quantitative determination of the concentration of H2O2 and glucose with a wide linear range. The detection limit for H2O2 and glucose based on the colorimetric method were as low as 84 nM and 0.41 μM, respectively. In contrast, the detection limit for H2O2 and glucose based on the fluorescent method were as low as 97 nM and 0.85 μM, respectively. Furthermore, the colorimetric readout on the paper device based on the changing color of the solution could also be integrated with a smartphone platform to conduct the on-site analysis of glucose without the use of the spectrometer. In addition, this dual sensor can be applied to detect glucose in real serum with highly accurate results, making it a good candidate for biosensor applications.
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