脂多糖
细菌外膜
免疫
微生物学
鲍曼不动杆菌
脾脏
免疫
生物
病毒学
免疫学
免疫系统
细菌
大肠杆菌
铜绿假单胞菌
基因
生物化学
遗传学
作者
Marta Pulido,Meritxell García-Quintanilla,Jerónimo Pachón,Michael J. McConnell
出处
期刊:Vaccine
[Elsevier]
日期:2020-01-01
卷期号:38 (4): 719-724
被引量:37
标识
DOI:10.1016/j.vaccine.2019.11.043
摘要
Outer membrane vesicles (OMVs) were isolated from an Acinetobacter strain deficient in lipopolysaccharide (LPS) due to a mutation in lpxD (IB010). Two immunizations with 10 µg of IB010 OMVs elicited total IgG, IgM, IgG1 and IgG2c titers similar to those observed after immunization with OMVs derived from the parental strain (ATCC 19606), and IB010 OMVs plus purified LPS. Immunization with IB010 OMVs resulted in significantly reduced post-infection spleen bacterial loads and serum IL-1β and IL-6 levels compared to control mice in a disseminated sepsis model. Mice immunized with 10 µg IB010 OMVs demonstrated significant, but partial, protection (75%) against infection, whereas mice immunized with ATCC 19606 OMVs or IB010 OMVs plus purified LPS were completely protected. Immunization of mice with 100 µg of IB010 OMVs completely protected mice from infection. This study demonstrates that LPS deficient A. baumannii produces OMVs, and that immunization with these OMVs elicits protective immunity against infection.
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