三乙氧基硅烷
表面改性
化学
材料科学
X射线光电子能谱
核化学
共轭体系
透射电子显微镜
傅里叶变换红外光谱
磁性纳米粒子
纳米颗粒
结合
化学工程
纳米技术
有机化学
聚合物
物理化学
数学分析
工程类
数学
作者
Diana Martínez-Matamoros,Socorro Castro-Garcı́a,Gabriela Ojeda Romano,Miguel Balado,Jaime Rodrı́guez,Manuel L. Lemos,Carlos Jiménez
摘要
In the present work, the synthesis of magnetic nanoparticles, its coating with SiO2, followed by its amine functionalization with (3-aminopropyl)triethoxysilane (APTES) and its conjugation with deferoxamine, a siderophore recognized by Yersinia enterocolitica, using a succinyl moiety as a linker are described. Magnetic nanoparticles (MNP) of magnetite (Fe3O4) were prepared by solvothermal method and coated with SiO2 (MNP@SiO2) using the Stöber process followed by functionalization with APTES (MNP@SiO2@NH2). Then, feroxamine was conjugated with the MNP@SiO2@NH2 by carbodiimide coupling to give MNP@SiO2@NH2@Fa. The morphology and properties of the conjugate and intermediates were examined by eight different methods including powder X-Ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and energy dispersive X-Ray (EDX) mapping. This exhaustive characterization confirmed the formation of the conjugate. Finally, in order to evaluate the capacity and specificity of the nanoparticles, they were tested in a capture bacteria assay using Yersinia enterocolitica.
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