驴子
多重聚合酶链反应
食品科学
熟肉
生肉
水牛肉
物种鉴定
琼脂糖凝胶电泳
生物
多路复用
聚合酶链反应
生物技术
DNA
基因
遗传学
生态学
作者
Muhammad Kashif Iqbal,Muhammad Sulyman Saleem,Muhammad Imran,Waseem Khan,Kamran Ashraf,Muhammad Zahoor,Imran Rashid,Habib-Ur Rehman,Asif Nadeem,Saadat Ali,Sarwat Naz,Wasim Shehzad
标识
DOI:10.1080/19393210.2020.1778098
摘要
Food adulteration has a direct impact on public health, religious faith, fair-trades, and wildlife. In the present study, a reliable and sensitive assay has been developed for verifying meat adulteration in food chain. The multiplex PCR system was optimised for identification of chicken, cow/buffalo, sheep/goat, horse/donkey, pork, and dog DNAs in a single reaction mixture simultaneously. The primers were designed using 12 S rRNA gene sequences with fragment size in the range of 113 bp to 800 bp, which can be easily visualised on agarose gel electrophoresis making the technique economical. After validation of accuracy, specificity, and sensitivity, commercially available meat products (n = 190) were screened, comprising both raw and cooked meat samples. The results demonstrated a high rate of adulteration (54.5%) in meat products. The technique developed here can be easily used for screening of different meat products for export and import purposes as well as for food inspection and livestock diagnostic laboratories.
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