核酸
适体
蜘蛛丝
化学
DNA
生物物理学
细胞内
亚细胞定位
纳米技术
丝绸
细胞生物学
生物化学
材料科学
生物
分子生物学
基因
复合材料
作者
Pai Peng,Yi Du,Jiao Zheng,Huihui Wang,Tao Li
标识
DOI:10.1002/anie.201811117
摘要
Abstract In nature, the formation of spider silk fibers begins with dimerizing the pH‐sensitive N‐terminal domains of silk proteins (spidroins) upon lowering pH, and provides a natural masterpiece for programmable assembly. Inspired by the similarity of pH‐dependent dimerization behaviors, introduced here is an i‐motif‐guided model to mimic the initial step of spidroin assembly at the subcellular level. A framework nucleic acid (FNA) nanoplatform is designed using two tetrahedral DNA nanostructures (TDNs) with different branched vertexes carrying a bimolecular i‐motif and a split ATP aptamer. Once TDNs enter acidic lysosomes within living cells, they assemble into a heterodimeric architecture, thereby enabling the formation of a larger‐size framework and meanwhile subcellular imaging in response to endogenous ATP, which can be dynamically manipulated by adjusting intracellular pH and ATP levels with external drug stimuli.
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