基因敲除
头颈部鳞状细胞癌
癌症研究
癌基因
组织微阵列
医学
细胞
细胞周期
微阵列分析技术
细胞凋亡
肿瘤科
癌症
生物
基因表达
内科学
基因
头颈部癌
生物化学
遗传学
作者
Yuli Fu,Guocai Tian,Zhiyuan Zhang,Xiao Yang
标识
DOI:10.1186/s12935-021-02394-w
摘要
Abstract Background Head and neck squamous cell carcinoma (HNSCC) are one of the most common types of head and neck cancer, and it is urgent to find effective treatment for advanced patients. Exploring developing and progressing mechanisms of HNSCC could provide a theoretical basis to find new therapeutic targets. Methods In our research, we performed a whole-gene expression profile microarray analysis to identify differential expression genes between squamous cell carcinoma cells and ΔNp63 alpha (ΔNp63α) knockdown cells. As a result, an important gene Synaptotagmin VII (SYT7) was screened out. Results SYT7 knockdown affected the proliferation, apoptosis and cell cycle of squamous cell carcinoma cells. The rescue experiment in vitro with ΔNp63α and SYT7 double knockdown resulted in partial reversion of ΔNp63α-induced phenotypes. This was also confirmed by experiments in vivo. Conclusions Taken together, we found that ΔNp63α could inhibit the occurrence and progression of HNSCC throughout downregulating the expression of SYT7. Therefore, SYT7/ΔNp63α axis could be a potential therapeutic target for clinical treatment of HNSCC.
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