Rapid Detection of Porcine Circovirus Type 2 by TaqMan-based Real-time Polymerase Chain Reaction Assays

PCV2 is the primary causative agent of porcine circovirus-associated disease (PCVAD). In this study, a TaqMan-based real-time polymerase chain reaction (PCR) assay targeting ORF2 gene of PCV2 was developed and their sensitivities and specificities were investigated. The results indicated that the standard curve had a wide dynamic range (102-107copies/µL) with a linear correlation (R2) of 0.997 between the cycle threshold (Ct) value and template concentration. The