枯草芽孢杆菌
原生质体
甘露醇
地衣芽孢杆菌
杆菌
明胶
琼脂
微生物学
生物
杆菌科
芽孢杆菌(形态)
芽孢杆菌目
转化(遗传学)
琼脂平板
生物化学
细菌
遗传学
基因
作者
Nigel Bourne,B. N. Dancer
出处
期刊:Microbiology
[Microbiology Society]
日期:1986-02-01
卷期号:132 (2): 251-255
被引量:6
标识
DOI:10.1099/00221287-132-2-251
摘要
Summary: Regeneration of protoplasts to bacilli was attempted in several strains of Bacillus closely related to Bacillus subtilis 168. On DM3 and similar media using succinate as osmotic support, only B. subtilis 168 and Bacillus natto ATCC 15245 were able to regenerate. Media containing mannitol as osmotic support, and agar as gelling agent gave rise to L-form colonies with Bacillus licheniformis NCTC 6346. Many of the L-form colonies were able to regenerate to the bacillary form when plated on the mannitol medium solidified with gelatin. All of the Bacillus species tested were able to regenerate on the latter medium at rates sufficient to allow protoplast transformation and fusion experiments.
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