发酵
甘油
1,3-丙二醇
短乳杆菌
生物反应器
化学
恒化器
产量(工程)
食品科学
生物柴油
工业发酵
色谱法
乳酸
细菌
生物化学
材料科学
催化作用
生物
有机化学
冶金
遗传学
植物乳杆菌
作者
Vivek Narisetty,T.V. Aswathi,Petit Riff Sven,Ashok Pandey,Parameswaran Binod
标识
DOI:10.1016/j.jbiotec.2017.07.033
摘要
The microbial conversion of biodiesel derived crude glycerol to 1,3-propanediol (1,3-PDO) has attained high industrial value due to the broad range of applications as a monomer (1,3-PDO) in textile, cosmetic and polymer industries. This fine chemical through biological production addressed several limitations of the chemical process like high temperatures, pressure and expensive catalysts. In this study 1,3-propanediol production was achieved from a non-pathogenic lactic acid bacterial strain Lactobacillus brevis N1E9.3.3 in suspended and immobilized form under batch, sequential batch and continuous modes of fermentation. The microorganism was immobilized in polyurethane foam cubes. The effects of initial glycerol concentration on suspended and immobilized cells were investigated in stirred tank reactor. The maximum 1,3-PDO titers of 51.5g1,3-PDO/l and 42.59g1,3-PDO/l with a yield of 0.64g1.3-PDO/gGlycerol and 0.53g1.3-PDO/gGlycerol using suspended and immobilized cells respectively was observed in batch fermentation with an initial glycerol concentration of 80g/l. In repeated batch (self-cycling) fermentation 78.3g/l 1,3-PDO with 0.65g1.3-PDO/gGlycerol yield and 0.65g/l/h productivity was observed at the end of 5th cycle. A constant yield between 0.3-0.65g1.3-PDO/gGlycerol was observed with cell recycling using suspended and immobilized cells. The highest productivity of 1.735g1,3-PDO/l/h was observed in chemostat with immobilized cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI