菁
化学
荧光
荧光团
光热治疗
量子产额
人血清白蛋白
摩尔吸收率
吲哚
体内
光化学
共轭体系
树枝状大分子
生物物理学
纳米技术
色谱法
材料科学
立体化学
有机化学
光学
聚合物
物理
生物技术
生物
作者
Peng-Fei Xu,Linan Hu,Cheng Yu,Wei-Dong Yang,Fei Kang,Mingru Zhang,Pei Jiang,Jing Wang
标识
DOI:10.1186/s12951-021-01075-0
摘要
Herein, an unprecedented synergistic strategy for the development of high-performance NIR-II fluorophore is proposed and validated. Based on an unsymmetrical cyanine dye design strategy, the NIR-II emissive dye NIC was successfully developed by replacing only one of the indoline donors of symmetrical cyanine dye ICG with a fully conjugated benz[c,d]indole donor. This minor structural change maximally maintains the high extinction coefficient advantage of cyanine dyes. NIC-ER with endogenous albumin-hitchhiking capability was constructed to further enhance its in vivo fluorescence brightness. In the presence of HSA (Human serum albumin), NIC-ER spontaneously resides in the albumin pocket, and a brilliant ~89-fold increase in fluorescence was observed. Due to its high molar absorptivity and moderate quantum yield, NIC-ER in HSA exhibits bright NIR-II emission with high photostability and significant Stokes shift (>110 nm). Moreover, NIC-ER was successfully employed for tumor-targeted NIR-II/PA imaging and efficient photothermal tumor elimination. Overall, our strategy may open up a new avenue for designing and constructing high-performance NIR-II fluorophores.
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