Insight into the effect of N-acyl-homoserine lactones-mediated quorum sensing on the microbial social behaviors in a UASB with the regulation of alkalinity

N-acyl-homoserine lactones (AHLs)-mediated quorum sensing (QS) has been reported as the inducers of microbial social behaviors in anaerobic digestion (AD) processes. However, it is not well understood that how to intentionally change the secretion of AHLs by conventional engineering control such as the regulation of alkalinity. The present research investigated the effect of endogenous AHLs-mediated QS on the microbial social behaviors in an upflow anaerobic sludge bed (UASB) reactor with the influent alkalinity decreased from 2800 mg/L to 700 mg/L by stages. The results showed that the alkalinity of 1800-2200 mg/L was more favorable for the AD in the UASB, with an excellent specific methanogenic activity (SMA) and better microbial aggregation statuses. The alkalinity out of the favorable alkalinity range would decrease the SMA with the accumulation of VFAs in the reactor. It was found that signal molecule C4-HSL was always the dominant AHL in the UASB along with the decrease of influent alkalinity, while 3-oxo-C6-HSL, 3-oxo-C12-HSL and C14-HSL were remarkably improved only within the favorable range of alkalinity. Pearson correlation concluded that the dominant signal molecule C4-HSL was the specific AHL in enhancing the synthesis of extracellular polysaccharide and the metabolism of acidogens. The co-occurrence network revealed that Mesotoga, Sulfurospirillum and Methanoregula were the key hubs in the microbial interaction network, and the AHLs-mediated QS indirectly facilitated the methanogenic metabolism. The present work provided a revealing insight into the effect of AHLs-mediated QS on the microbial social behaviors in AD process with the regulation of alkalinity.