纳米笼
化学
级联
材料科学
生物催化
纳米技术
葡萄糖氧化酶
纳米颗粒
酶
催化作用
组合化学
生物传感器
生物化学
色谱法
离子液体
作者
Qiuping Wang,Min Chen,Can Xiong,Xiaofei Zhu,Chen Cai,Fangyao Zhou,Yun Dong,Yu Wang,Jie Xu,Yimin Li,Jing Liu,Hongjun Zhang,Bangjiao Ye,Huang Zhou,Yuen Wu
标识
DOI:10.1016/j.bios.2021.113695
摘要
The intrinsically fragile nature and leakage of the enzymes is a major obstacle for the commercial sensor of a continuous glucose monitoring system. Herein, a dual confinement effect is developed in a three dimensional (3D) nanocage-based zeolite imidazole framework (NC-ZIF), during which the high-loading enzymes can be well encapsulated with unusual bioactivity and stability. The shell of NC-ZIF sets the first confinement to prevent enzymes leakage, and the interior nanocage of NC-ZIF provides second confinement to immobilize enzymes and offers a spacious environment to maintain their conformational freedom. Moreover, the mesoporosity of the formed NC-ZIF can be precisely controlled, which can effectively enhance the mass transport. The resulted GOx/Hemin@NC-ZIF multi-enzymes system could not only realize rapid detection of glucose by colorimetric and electrochemical sensors with high catalytic cascade activity (with an 8.3-fold and 16-fold enhancements in comparison with free enzymes in solution, respectively), but also exhibit long-term stability, excellent selectivity and reusability. More importantly, the based wearable sweatband sensor measurement results showed a high correlation (>0.84, P < 0.001) with the levels measured by commercial glucometer. The reported dual confinement strategy opens up a window to immobilize enzymes with enhanced catalytic efficiency and stability for clinical-grade noninvasive continuous glucose sensor.
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