A sequentially activated bioluminescent probe for observation of cellular H2O2 production induced by cysteine

We report herein a caged luciferin probe Cy-Hy as a sequentially activated probe to selectively and sensitively sense l -Cys and H 2 O 2 , utilizing the present probe, the cellular excess l -Cys induced H 2 O 2 up-regulation was observed for the first time in the living MDA-MB-231 cells.