Inhibitory effect of paclitaxel on proliferation of vascular smooth muscle cell in rats

Objectives To evaluate the inhibitory effect of paclitaxel on proliferation of vascular smooth muscle cell in rats. Methods Isolated the aortae of the rats, drawed off the smooth muscle and cut them into fragment, then put the fragment in the culture flask to cultivate. Verificated the cells by inverted aberratio microscope and α-smooth muscle-actin immunofluorescence detection after they have been passaged for 4-6 times. The smooth muscle cells were divided into 4 different groups according to the adding medicine: group A with non serum M199 medium; group B with 1 nmol / L paclitaxel, group C with 10 nmol / L paclitaxel and group D with 100 nmol / L paclitaxel. There were 5 samples in each group. Flow cytomery was used to detect the grow periodicity of the smooth muscle cells and vascular smooth muscle cell proliferating cell nuclear antigen (PCNA) were also detected. Results A great quantity of cells growed after 2 weeks. The cells were observed fusiform shape and presented peak-valley grow phenomenon by inverted aberration microscope. The α-smooth muscle-actin immunofluorescence detection of the culture smooth muscle cells were positive and the positive rate was over 90%. The posititve rate of the smooth muscle cell PCNA presented a taper tendency, following the increase dosage of paclitaxel. Compared with group A, the posititve rate of the smooth muscle cell PCNA in group C and group D decreased significantly. The flow cytometry results indicated that the S period rate of the smooth muscle cell presented a taper tendency, following the increase dosage of paclitaxel. Compared with group A, the S period rate in group C and group D decreased significantly. Conclusions Paclitaxel can inhibit the proliferation of vascular smooth muscle cell in rats.