Determination of Crocin I and Crocin II Sim Haneovs by HPLC

Objective To establish a method for determination of crocin Ⅰ and crocin Ⅱ simultaneously by HPLC. Methods Elite C8 chromatographic column(4.6 mm×250mm,5μm) was used,the mobile phase was methanol water acetic acid(75∶24.5∶0.5),the detection wavelength was 440nm,and the flow rate was 0.6 ml·min-1. Results Under this condition,crocin Ⅰ and crocin Ⅱ got better separation,both the contents of crocin Ⅰ and crocin Ⅱ and their peak areas were in good linear relationships respectively,the average recovery of this method was 102.13%. Conclusion This method is repeatable,quantitative,accurate,rapid,and effective for the simultaneous determination of crocin Ⅰ and crocin Ⅱ.