Effects of~(60)Co-γ ray on rice anther culture breeding

The results of applying 60 Co γ ray to rice anther culture showed that the frequencies of the calli induction and the green plantlet differentiation increased when anthers was treated with 5 10 Gy of gamma ray before inoculation.The cultured ability could not increase but the variation frequencies of anther green plants increased.The anther culture ability of MF 1 anthers from F 0 seeds irradiated with 150 200Gy of gamma ray would lower down.It was not very beneficial to induction of calli when the dose of irradiation was over 250Gy.