Study on the Enzymatic Hydrolysis of Spirulina Protein

[Objective] The aim of the research was to study the hydrolysis of papain on spirulina protein.[Method] The effects of temperature,enzyme concentrate,pH value,time on enzymatic hydrolysis were studied by orthogonal design.The molecular weight of the peptide was determind using sephadex G-25.[Result] The optimum technological parameters of the hydrolysis of papain on spirulina protein was,temperature 55 ℃,enzyme-substrate ratio 0.4%,time 1 h,pH value 6.0.[Conclusion]When the degree of hydrolysis was 82.6%,the molecular weights of two elution peak of peptides were 2 992 and 231 Da.