Separation puerarin from Pueraria lobata by the method of Fe~(3+) complex extraction

A new type of separating puerarin from Chinese traditional medicine Pueraria lobata method was found by using the property of Fe 3+ complex with puerarin.Pueraria isoflavonid was macerated with methanol,and was hydrolyzed with the solution of HCl.After the solution of hydrolyzing was neutralized,FeCl 3 was put into it.Puerarin was coordinated with Fe 3+ and dissolved.Filtrated the others infusibility constituents,the complex of Fe 3+ puerarin in the filtrate was divided by HCl and the puerarin was obtained.Meanwhile,the best acidity of division Fe 3+ puerarin complex was determined by the method of Spectrophotometry.The product of puerarin was determined by the mehod of HPTLC,IR and Spectrophotometry,respectively.It was a convenient and efficient way to extract Pueraia isoflavonid from P.lobata .The recovery and purity of puerarin was 1.2% and 95.6% ,respectively.