Effect of 2,4-D on Somatic Embryogenesis in Mature and Immature Zygotic Embryo of Panax quinquefolium

The mature and immature Panax quinquefolium seeds were used as test materials,and the zygotic embryo were dissected out aseptically and placed on solid MS medium supplemented with different concentrations of 2,4-D in the light and dark culture conditions,effect of 2,4-D on the somatic embryogenesis of american ginseng were studied.The results showed that somatic embryogenesis could occur on the mature embryos at different concentrations of 2,4-D and the optimal concentration were 0.5 mg/L,and illumination could stimulate the process;The immature embryo could form somatic embryo.The conclusion were that 2,4-D stimulated somatic embryogenesis on the mature embryos,the responses between mature and immature embryo were apparently different;The developmental control mechanism in zygotic embryo of Panax quinquefolium was from that in Panax ginseng.