肾单位
细胞骨架
亚细胞定位
锚定
粘合连接
细胞生物学
生物
钙粘蛋白
肾
共域化
细胞质
生物物理学
细胞
生物化学
内分泌学
基因
作者
Peter Piepenhagen,Luanne L. Peters,Samuel E. Lux,W. James Nelson
出处
期刊:American Journal of Physiology-cell Physiology
[American Physiological Society]
日期:1995-12-01
卷期号:269 (6): C1417-C1432
被引量:63
标识
DOI:10.1152/ajpcell.1995.269.6.c1417
摘要
Ionic homeostasis in vertebrates is maintained by epithelial cells that line kidney nephrons. Transport of ions and solutes is coupled to Na+ reabsorption from the ultrafiltrate and requires specific subcellular distribution and activity of Na(+)-K(+)-ATPase along the nephron. Studies using cell culture models of renal epithelia indicate that the subcellular distribution of Na(+)-K(+)-ATPase is regulated by interactions with the submembrane cytoskeleton and E-cadherin-mediated adherens junctions. We have now examined the relevance of these in vitro observations to the subcellular organization of these proteins in different nephron segments of the adult mouse kidney using immunofluorescence microscopy. Our results demonstrate that segmental and subcellular distributions of Na(+)-K(+)-ATPase and the membrane-cytoskeletal proteins, ankyrin and fodrin, vary in parallel along the nephron and do not parallel variations in expression of the tight junction protein ZO-1 or E-cadherin. These data indicate that a mechanism for restricting Na(+)-K(+)-ATPase subcellular distributions through interactions with the membrane cytoskeleton is likely to be relevant in vivo.
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