The Cytomorphology Study of Anther Abortion in Male-Sterile Lines of Hong Cai-tai (Brassica campestris L.ssp.chinensis L.var.utilis Tsen et Lee.)

Microsporogenesis of Hong Cai-tai's Polima cytoplasmic male-sterile line (Polima CMS),Hong Cai-tai's Ogura cytoplasmic male-sterile line(Ogura CMS) and their maintainers were investigated cytologically by using paraffin section with light microscopy systemly.The observation showed that the anther development of Hong Cai-tai's Polima male-sterile line was blocked without anther at the stage of archesporial cell.The few pollen and no anther cell was observed without forming the tapetum and the middle layer;while the anther development of Hong Cai-tai's Ogura male-sterile line was blocked without anther at the stage of microspore mother cell tetrads of microspores,observing the tapetum cell abnormal and tetrads of microspores being extruded.In the end the tapetum cell and tetrads of microspores disaggregated.