Relationship between the glioma edema in human brain and AQP4

Objective To investigate the expression of aquaporin 4 (AQP4) and its regulation in human brain glioma and to elaborate the mechanism of occurrence and development of glioma edema.Methods The expression of AQP4mRNA and AQP4 protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western-Blot analysis in 24 cases of gliomas.Results By semiquantitative RT-PCR and Western-Blot analysis,the expression of AQP4mRNA and AQP4 protein in glioma got greater increase than that of the control group.There was significant difference between the two groups (P0.01).Conclusion The expression of AQP4mRNA and AQP4 protein in glioma is highly positively related to the malignant grade of glioma.It has been proved that AQP4 takes part in the process of glioma edema and plays an important role in process of glioma edema.