Extracts of phenolic compounds were obtained from different blueberry cultivars, Vaccinium corymbosum (Elliot, BlueCrop, and Duke) and genotypes, Vaccinium myrtillus (Wild 1, Wild 2). The content of anthocyanin aglycons was determined after acid hydrolysis using RP-HPLC-DAD. The antioxidant activity of the obtained extracts was also investigating using a new method, HPS. Vaccinium myrtillus , both wild cultivars had high amount of anthocyanins (940 mg/100 g FW, respectively 531.54 mg/100 g FW) comparing to cultivated ones. The lowest values were obtained for Duke variety (100, 9 mg/100 g FW). In agreement with these results, antioxidant activity showed similar behavior between the samples. Until now the antioxidant method (HPS) was tested for the first time for blueberry varieties.