Study on the bacteriostatic property and bacteriostatic mechanism of rosemarinic acid compounded bacteriostatic agent

In this study, a composite bacteriostatic agent (0.1250 g/L rosemarinic acid and 0.0555 g/L citral) was prepared to enhance the utilization of rosemarinic acid by synergistically making it more effective against common contaminating bacteria. The mechanism of bacterial inhibition was analyzed using GC-MS technology and metabolomics. The results showed that rosemarinic acid was able to affect the energy metabolism of bacteria, synergized with citral to cause the accumulation of ketone bodies in E. coli (No. 1.12883) and affect the TCA cycle, as well as disrupt the cell membrane of S. aureus (No. 1.282). The results provide ideas for the development of novel antibiotics.