A dual-response fluorescent probe Rh-O-QL for simultaneous monitoring of NAD(P)H and pH during mitochondrial autophagy

Mitochondrial autophagy is closely related to abnormal NAD(P)H and pH. Here, we synthesized a dual-response fluorescent probe with high selectivity for NAD(P)H and sensitivity in the physiological pH range, for simultaneous imaging analysis of mitochondrial NAD(P)H and pH, holding potential as a novel tool for understanding mitochondria-associated diseases.