化学
微分脉冲伏安法
生物传感器
检出限
电化学
循环伏安法
图层(电子)
电化学气体传感器
纳米技术
逐层
分析化学(期刊)
色谱法
电极
生物化学
有机化学
材料科学
物理化学
作者
Dujuan Li,Huang Wei,Rui Hong,Xiaojie Yue,Linxi Dong,Kai Fan,Yu Jing,Dong-Lai Yao,Hong Xu,Jun Lu,Gaofeng Wang
出处
期刊:Talanta
[Elsevier]
日期:2024-07-01
卷期号:274: 125965-125965
标识
DOI:10.1016/j.talanta.2024.125965
摘要
In this paper, a few-layer WS2 nanosheets-based electrochemical biosensor was fabricated for the highly sensitive detection of breast cancer tumor marker miRNA-4484. Firstly, few-layer WS2 nanosheets were prepared by shear stripping and characterized by SEM, TEM, AFM and UV spectrophotometer. After modification of few-layer WS2 nanosheets on the electrode surface, the miRNA probe was fixed on the few-layer WS2 nanosheets by polycytosine (PolyC). Then short-chain miRNA containing PolyC was used as the blocking agent to close the excess active sites on the surface of WS2 nanosheets to complete the fabrication of the sensor biosensing interface. Finally, the current changes caused by the specific binding of miRNA-4484 to the probe were analyzed by differential pulse voltammetry (DPV). The results showed that the sensor had a good linear relationship for the detection of miRNA-4484 in the concentration range of 1 aM-100 fM, and the detection limit was as low as 1.61 aM. In addition, the electrochemical sensor had excellent selectivity, stability and reproducibility. The artificial sample tests indicated that the developed biosensors have the potential for clinical application in the future.
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